2012, ISBN: 9783659219689

As the most important molecules, proteins often undergo post-translational modifications (PTM) to carry out their functions. In my study, two methods have been developed for the synthesis… Más…

As the most important molecules, proteins often undergo post-translational modifications (PTM) to carry out their functions. In my study, two methods have been developed for the synthesis of proteins containing PTMs. The first method is chemical ligation at valine residue. The thioacid capture ligation was found to be more efficient to overcome steric hindrance of the tertiary thiol group in penicillamine compared with thioester-mediated ligation. The second method is a direct and easy-to-use approach to introducing site-specific acetylation onto recombinant proteins. The free thiol of cysteine was reacted with N-vinylacetamide (NVA) by radical-mediated thiol-ene coupling to generate acetyl-4-thialysine (sLys (Ac)) which is anacetyl-lysine analog. In the last part of this work, we have also introduced a new strategy for selective disulfide formation directed by PNA pairing. This method can be more useful than the DNA-templated reaction systems. Inhoud: Taal: en; Bindwijze: Paperback; Oorspronkelijke releasedatum: 30 augustus 2012; Aantal pagina's: 152; Illustraties: Nee; Betrokkenen: Hoofdauteur: Fupeng Li; Hoofduitgeverij: Lap Lambert Academic Publishing; Overige kenmerken: Extra groot lettertype: Nee; Gewicht: 231 g; Product breedte: 152 mm; Product hoogte: 9 mm; Product lengte: 229 mm; Studieboek: Ja; Verpakking breedte: 152 mm; Verpakking hoogte: 9 mm; Verpakking lengte: 229 mm; EAN: Engels | Paperback | 9783659219689 | 30 augustus 2012 | 152 pagina's, Boeken, Wetenschap & Natuur, Biologie, Lap Lambert Academic Publishing<

2012, ISBN: 3659219681

[EAN: 9783659219689], Nieuw boek, [SC: 14.0], [PU: LAP LAMBERT Academic Publishing], nach der Bestellung gedruckt Neuware - Printed after ordering - As the most important molecules, prote… Más…

[EAN: 9783659219689], Nieuw boek, [SC: 14.0], [PU: LAP LAMBERT Academic Publishing], nach der Bestellung gedruckt Neuware - Printed after ordering - As the most important molecules, proteins often undergo post-translational modifications (PTM) to carry out their functions. In my study, two methods have been developed for the synthesis of proteins containing PTMs. The first method is chemical ligation at valine residue. The thioacid capture ligation was found to be more efficient to overcome steric hindrance of the tertiary thiol group in penicillamine compared with thioester-mediated ligation. The second method is a direct and easy-to-use approach to introducing site-specific acetylation onto recombinant proteins. The free thiol of cysteine was reacted with N-vinylacetamide (NVA) by radical-mediated thiol-ene coupling to generate acetyl-4-thialysine (sLys (Ac)) which is anacetyl-lysine analog. In the last part of this work, we have also introduced a new strategy for selective disulfide formation directed by PNA pairing. This method can be more useful than the DNA-templated reaction systems. 152 pp. Englisch, Books<

2012, ISBN: 3659219681

[EAN: 9783659219689], Neubuch, [PU: LAP LAMBERT Academic Publishing], nach der Bestellung gedruckt Neuware - Printed after ordering - As the most important molecules, proteins often under… Más…

[EAN: 9783659219689], Neubuch, [PU: LAP LAMBERT Academic Publishing], nach der Bestellung gedruckt Neuware - Printed after ordering - As the most important molecules, proteins often undergo post-translational modifications (PTM) to carry out their functions. In my study, two methods have been developed for the synthesis of proteins containing PTMs. The first method is chemical ligation at valine residue. The thioacid capture ligation was found to be more efficient to overcome steric hindrance of the tertiary thiol group in penicillamine compared with thioester-mediated ligation. The second method is a direct and easy-to-use approach to introducing site-specific acetylation onto recombinant proteins. The free thiol of cysteine was reacted with N-vinylacetamide (NVA) by radical-mediated thiol-ene coupling to generate acetyl-4-thialysine (sLys (Ac)) which is anacetyl-lysine analog. In the last part of this work, we have also introduced a new strategy for selective disulfide formation directed by PNA pairing. This method can be more useful than the DNA-templated reaction systems. 152 pp. Englisch, Books<

2012, ISBN: 3659219681

Kartoniert / Broschiert, met couverture 11, [PU:LAP LAMBERT Academic Publishing]

2012, ISBN: 3659219681

[EAN: 9783659219689], Neubuch, [PU: LAP Lambert Academic Publishing 2012-08], Books